SPECIALIZED EQUIPMENT

 

Modern animal facility - operating in SPF standard; houses several strains of knockout and transgenic mice and rats, kept in individually ventilated cages (IVC). The facility is equipped in:

 

  • IVIS© Lumina II (Caliper Life Science) - imaging system for both fluorescent and bioluminescent imaging in vivo;
  • Exer 3/6 (Columbus Instruments) - animal treadmill for mice and rats;
  • scil Vet abc (Horiba) – hematological and biochemical analyzer (for analyzing blood of various animal species);
  • Aurora1300A: 3-in-1 Whole Animal System (Aurora Scientific) – system for the measurement of rodent muscle properties in situ, in vivo and in vitro;
  • Micromanipulator;
  • Grip Strength Meter (Ugo Basile) - a system for determining the gripping strength (the maximum force) of small laboratory animals (rat, mouse);
  • scil Vet abc – hematology analyzer (different animal species can be analyzed);
  • SPOTCHEM EZ SP-4430 (Arkray) - automated analyzer for clinical chemistry;
  • IBIDI Pump system -organ-on-a-chip equipment.

 

Flow cytometry laboratory equipped in:

  • BD LSRFortessa (BD Biosciences) - flow cytometer;
  • MoFloTM XDP (Beckman Coulter) - high-speed cell sorter;

 

Histological laboratory equipped in:

  • Automated tissue processor;
  • Automated tissue embedding system;
  • Microtome - with the Section-Transfer-System;
  • Cryostat;
  • Automated staining system for paraffin sections;
  • Laser Microdissection LMD7000 (Leica).

 

Other equipment:

  • Chambers for hypoxic conditions/hypoxic incubators/hypoxic laminar for cells culturing in low oxygen concentration;
  • Bioanalyzer 2100 (Agilent) for checking the RNA integrity;
  • Real-time PCR StepOnePlus (Applied Biosystem) for analysis of a gene expression profile on mRNA level, as well as microRNAs analysis;
  • Multiwell-MEA-System – measurement of electrophysiological activity of cells
  • TissueLyser II – mechanical tissue grinding
  • Automatic wire myograph system - 630MA enabling examination of the vascular function of any vessel segment
  • ChemiDoc Imaging Systems for fast and sensitive detection of the chemiluminescent signal in western blots.