SPECIALIZED EQUIPMENT
Modern animal facility - operating in SPF standard; houses several strains of knockout and transgenic mice and rats, kept in individually ventilated cages (IVC). The facility is equipped in:
- IVIS© Lumina II (Caliper Life Science) - imaging system for both fluorescent and bioluminescent imaging in vivo;
- Exer 3/6 (Columbus Instruments) - animal treadmill for mice and rats;
- scil Vet abc (Horiba) – hematological and biochemical analyzer (for analyzing blood of various animal species);
- Aurora1300A: 3-in-1 Whole Animal System (Aurora Scientific) – system for the measurement of rodent muscle properties in situ, in vivo and in vitro;
- Micromanipulator;
- Grip Strength Meter (Ugo Basile) - a system for determining the gripping strength (the maximum force) of small laboratory animals (rat, mouse);
- scil Vet abc – hematology analyzer (different animal species can be analyzed);
- SPOTCHEM EZ SP-4430 (Arkray) - automated analyzer for clinical chemistry;
- IBIDI Pump system -organ-on-a-chip equipment.
Flow cytometry laboratory equipped in:
- BD LSRFortessa (BD Biosciences) - flow cytometer;
- MoFloTM XDP (Beckman Coulter) - high-speed cell sorter;
Histological laboratory equipped in:
- Automated tissue processor;
- Automated tissue embedding system;
- Microtome - with the Section-Transfer-System;
- Cryostat;
- Automated staining system for paraffin sections;
- Laser Microdissection LMD7000 (Leica).
Other equipment:
- Chambers for hypoxic conditions/hypoxic incubators/hypoxic laminar for cells culturing in low oxygen concentration;
- Bioanalyzer 2100 (Agilent) for checking the RNA integrity;
- Real-time PCR StepOnePlus (Applied Biosystem) for analysis of a gene expression profile on mRNA level, as well as microRNAs analysis;
- Multiwell-MEA-System – measurement of electrophysiological activity of cells
- TissueLyser II – mechanical tissue grinding
- Automatic wire myograph system - 630MA enabling examination of the vascular function of any vessel segment
- ChemiDoc Imaging Systems for fast and sensitive detection of the chemiluminescent signal in western blots.